MiR-423-5p downregulates osteoblastic differentiation and cell viability by targeting SMAD3 in non-traumatic osteonecrosis


 Purpose: To investigate the potential role of miR-423-5p in osteoblastic differentiation non-traumatic osteonecrosis femoral head (ONFH).
 Methods: MiR-423-5p levels bone marrow samples from ONFH and osteoarthritis (OA) patients, respectively, were evaluated using quantitative (real-time) polymerase chain reaction (qPCR). Osteoblastic was monitored Alizarin red S staining, while cell viability determined by MTT assay hMSC-BM. expression also measured during differentiation. The underlying mechanisms explored TargetScan database, a series vitro experiments performed to confirm this.
 Results: significantly upregulated (p < 0.01) increased human mesenchymal stem cells-bone (hMSC-BM) after morphogenetic protein 2 (BMP-2) treatment. Furthermore, downregulated suppressed viability. SMAD3 observed be downstream target via bioinformatics analysis; further confirmed Conclusion: downregulates targeting osteonecrosis. Thus, may serve as for promoting patients.